Dot dema (Colletotrichum coccodes) chave chirwere chembatatisi chakakosha mune zvehupfumi nekuda kwekugona kwayo kukonzera kufa kwemashizha. Chirwere chinonzi black dot chine chekuita neakawanda black microsclerotia inogona kubuditswa nefungus paari . Muna Nyamavhuvhu-Gunyana 2022, dema dot zviratidzo zvakaonekwa pa c. 48% yemadzinde embatatisi (ingori pamusoro pevhu) , c. 27% ye stolons uye c. 22% yeganda remachubhu akakohwewa muminda yakashama [Goodlands (20°04'13.1“S 57°39'37.0”E), Réduit (20°14'00.5“S 57°29'28.5”E) nePlaine Sophie (20°22'23.0“S 57°29'08.4”E)] muMauritius.
Matishu gumi neshanu embatatisi ane chirwere akachekwa kuita zvidimbu zvisere pachishandiswa murazvo-sterilized cork-borer, izvo zvakabva zvaiswa disinfected ne 10% bleach kwemaminetsi maviri, zvichiteverwa nekusukurudzwa nemvura isina tsvina yakasvibiswa katatu, uye pakupedzisira yakaiswa pa chloramphenicol-yakagadziridzwa. mbatatisi dextrose agar (PDA) ndiro. Mahwendefa akaiswa incubated pa25 ° C kwemazuva gumi murima. White colonies ine flat uye hyaline mycelia yakaonekwa pamahwendefa ese akaiswa iyo yakazogadzira yakawanda yakasviba uye spherical sclerotia pakoloni mumazuva gumi. Tsika dzakachena dzakawanikwa nehyphal tip kuendeswa kuPDA, yakavharirwa kwemazuva gumi pa 10 ° C murima, uye yakaongororwa nokuda kwemaitiro e morphological. Kutevera kusvibiswa nedonje lactophenol dhayi uchishandisa yakachena siraidhi tekinoroji, akawanda, madiki, akaomeswa zvishoma uye unicellular conidia akaonekwa aine kureba nekufara.n = 50) ye11.0 uye 3.6 μm zvakateerana .
Iyo pathogenicity yeimwe yeatatu akaparadzana (P200) yakasimbiswa pamatanho matanhatu emazuva makumi mapfumbamwe ematatisi uye machubhu. Mbatatisi dzembatatisi dzine hutano dzakatanga kunyudzwa nekunyudzwa mu90% sodium hypochlorite kwemaminetsi maviri, yosukurudzwa kaviri mumvura ine isina tsvina uye yakasiiwa kuti iome mumapepa ane sterilized tisati tadyara mabhegi epurasitiki ega ega (2 litita). Mushure memazuva makumi mana neshanu, mbesa dzembatatisi dzakadiridzwa ne2 mL yekonidial suspension (45).6 conidia/ml) yekuzvitsaura P200 padyo nenzvimbo yekororo. Miti isina-inoculated mbatatisi yakashanda sekutonga uye yakadiridzwa nemvura ine sterile distilled. Mushure memazuva makumi mapfumbamwe, ongororo yekuona yakaratidza zviratidzo zvemadota matema pazasi pemadzinde, pedyo nevhu. Machubhu akakohwewa aive nemavara mapfumbu aine sclerotia paganda. Kudzora mbatatisi zvirimwa hazvina kuratidza zviratidzo pamazinde kana machubhu akakohwewa. Zvitsauko zvepakutanga zvakadzorerwa kubva kune inoculated tubers, nokudaro kuzadzisa Koch's postulates. Kuedza kwakadzokororwa katatu nemigumisiro yakafanana.
DNA yakatorwa kubva kune gumi-mazuva ekare tsika dzevatatu vakazvitsaura vachishandisa CTAB protocol. PCR amplification uye sequencing zvakaitwa neITS10/ITS5 uye GDF/GDR primer pairs for the internal transcribed spacer (ITS) uye glyceraldehyde 4-phosphate dehydrogenase regions, zvichiteerana. Kutsvaga kweBLASTn kwakaitwa nekutevedzana kwega rega rega, P3 (ITS: GenBank Accession No. OR86; GADPH: OR145915), P232172 (ITS: OR136; GADPH: OR145916) uye P232173 (ITS200: 145917: OR232174: GADPH99;100). Kutevedzana kwese kwaive ne100% kusvika XNUMX% kuzivikanwa nepamusoro peXNUMX Colletotrichum coccodes inozviparadzanisa nevamwe, semuenzaniso 100% identity ine yakazvimiririra BBA 70879 (MT221566.1) yeITS , uye 99.66% kuzivikanwa ine yega CBS 112987 (JX546735) yeGADPH .
Sekuziva kwedu, uyu ndiwo mushumo wekutanga weblack dot pambatatisi inokonzerwa ne C. makokodhe muMauritius. Zvakabuda muongororo iyi zvichabatsira kuwedzera ruzivo rwemumiririri uyu uye kubatsira varimi vezvekurima panguva yekuongorora zvirwere muminda yembatatisi.
Reference: Takoree, SD, Neetoo, H. & Ranghoo-Sanmukhiya, VM (2023) Chirevo chekutanga che Colletotrichum coccodes kukonzera dot dema pambatatisi muMauritius. Mishumo Yezvirwere Zvitsva, 48, e12224. https://doi.org/10.1002/ndr2.12224
